Genomics research and education initiatives
Rays Jiang is one of the founding members of USF genomics program; her lab bears the identity of STEM_CORE (Single-cell, Translational, Evolution, Microbial, USF Central-lab of Omics Research and Engineering).
Rays Jiang is the concentration director of the Genomics Master of Science (certified and approved) at USF.
Featured publications
*co-first authorship, ^co-senior authorship
Sacco MD, Wang S, Adapa SR, ... Gelis I, Jiang RHY, Sun X, Chen Y.
A unique class of Zn2+-binding serine-based PBPs underlies cephalosporin resistance and sporogenesis in Clostridioides difficile.
Nature Communications 2022 PMID: 35902581
Zhang M*, Wang C*, Oberstaller J*, Thomas P, Otto TD, Casandra D, Boyapalle S, Adapa SR, Xu S, Button-Simons K, Mayho M, Rayner JC, Ferdig MT, Jiang RHY, Adams JH.
The apicoplast link to fever-survival and artemisinin-resistance in the malaria parasite.
Nature Communications 2021 PMID: 34315897
Zhang M*, Wang CQ*, Otto TD, Oberstaller J, Liao X, Adapa SR, Udenze K, Li S, Bronner IF, Casandra D, Mayho M, Brown J, Li S, Swanson J, Rayner JC, Jiang RHY^ and Adams JH^.
Uncovering the essential genome of the human malaria parasite Plasmodium falciparum by saturation mutagenesis
Science 2018 PMID: 29724925
This work is featured by NIH Director Collins' post
Brancucci NMB, Gerdt JP, Wang C, ...Voss TS, Waters AP, Jiang RHY,
Clardy J, Marti M.
Lysophosphatidylcholine Regulates Sexual Stage Differentiation
in the Human Malaria Parasite Plasmodium falciparum.
Cell 2017 PMID: 29129376
Selected recent publications
Cloud-based and community-based open source discoveries
Adapa SR*, Taylor RA*, Wang C, Thomson-Luque R, Johnson LR, Jiang RHY.
Plasmodium vivax readiness to transmit: implication for malaria eradication.
BMC Systems Biology 2019 PMID: 30634978
Van Voorhis et al. Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond.
PLoS Pathogen 2016 PMID: 27467575
Single-cell engineering, computation and biology
Thomson-Luque R, Wang C, ..., Conway A, Adapa SR,Barnes SJ, Adams JH, Jiang RHY.
In-depth phenotypic characterization of reticulocyte maturation using mass cytometry.
Blood Cells Mol Dis. 2018 PMID:
30007855
This is the first single-cell human reticulocyte and erythroid study
Cheemalapati SV, Winskas J, Wang H, Konnaiyan K, Zhdanov A, Roth A, Adapa SR,
Deonarine A, Noble M, Das T, Gatenby R, Westerheide SD, Jiang RHY and Pyayt A.
Subcellular and in-vivo Nano-Endoscopy.
Scientific Report 2016 Oct
Novel computational methods and analysis
Kazi A, Xiang S, Yang H, Delitto D, Trevino J, Jiang RHY, Ayaz M, Lawrence HR,
Kennedy P, Sebti SM.
GSK3 suppression upregulates β-catenin and c-Myc to abrogate
KRas-dependent tumors.
Nature Communications 2018 PMID: 30514931
Gibbons J, Button-Simons KA, Adapa SR, Li S, Pietsch M, Zhang M, Liao X, Adams
JH, Ferdig MT, Jiang RHY.
Altered expression of K13 disrupts DNA replication and
repair in Plasmodium falciparum.
BMC Genomics 2018 PMID: 30486796
Babatunde KA, Mbagwu S, Hernández-Castañeda MA, Adapa SR, Walch M, FilgueiraL, Falquet L, Jiang RHY, Ghiran I, Mantel PY.
Malaria infected red blood cells release small regulatory RNAs through extracellular vesicles.
Scientific Report 2018 PMID: 29343745
Wang CQ, Adapa SR, Gibbons J, Sutton SG and Jiang RHY
Punctuated chromatin states regulate Plasmodium falciparum antigenic variation at intron and 2kb upstream regions
BMC Genomics 2016 Aug
Large scale of novel forward mutagenesis
Bronner IF, Ottoa TD, Zhang M, Udenze K, Wang CQ, Quail MA, Jiang RHY, Adams
JH, Rayner JC.
Quantitative Insertion-site Sequencing (QIseq) for high throughput
phenotyping of transposon mutants.
Genome Research. 2016 May 10
Egan ES, Jiang RHY, Moechtar MA, Barteneva NS, Weekes MP, et al
A forward
genetic screen identifies erythrocyte CD55 as essential for Plasmodium falciparum
invasion.
Science 2015 ; 348(6235):711-4
Human evolution driven by infectious diseases
Dankwa S, Lim C, Bei AK, Jiang RHY, Abshire JR, Patel SD, Goldberg JM, et al.
Ancient human sialic acid variant restricts an emerging zoonotic malaria parasite.
Nature Communications 2016 Apr 4
Dankwa S, Chaand M, Kanjee U, Jiang RHY, Nobre LV, et al.
Genetic Evidence for Erythrocyte Receptor Glycophorin B Expression Levels Defining a Dominant Plasmodium falciparum Invasion Pathway into Human
Erythrocytes.
Infection & Immunity 2017 PMID: 28760933
Mirobial evolution driven by hosts and environments
Jiang RHY, Stahelin RV, Bhattacharjee S, Haldar K. Eukaryotic virulence
determinants utilize phosphoinositides at the ER and host cell surface.
Trends in Microbiology. 2013 Mar;21(3):145-56
Jiang RHY and Tyler BM
Mechanisms and evolution of virulence in oomycetes
Annu Rev Phytopathol 2012 Vol 50 Sep 8;50:295-318 PMID: 22920560
Neafsey DE, Galinsky K, Jiang RHY, Young L, Sykes SM et al.
The malaria parasite Plasmodium vivax exhibits greater genetic diversity than Plasmodium falciparum.
Nature Genetics 2012 Sep;44(9):1046-50.
Mechanisms of host-microbe interactions
Ganter M, Goldberg JM, Dvorin JD, Paulo JA, King JG, Tripathi AK, Paul AS, Yang J, Coppens I, Jiang RHY, Elsworth B, Baker DA, Dinglasan RR, Gygi SP, Duraisingh MT.
Plasmodium falciparum CRK4 directs continuous rounds of DNA replication during schizogony.
Nature Microbiology 2017 Feb
Pellé KG*, Jiang RHY*, Mantel PY, Xiao YP, Hjelmqvist D, Gallego-Lopez GM, Lau
AO, Kang BH, Allred DR, Marti M.
Shared elements of host-targeting pathways among
apicomplexan parasites of differing life styles.
Cell Microbiol 2015; May 21
Paul AS, Saha S, Engelberg K, Jiang RHY, Coleman BI, Kosber AL, et al
Parasite Calcineurin Regulates Host Cell Recognition and Attachment by Apicomplexans.
Cell Host Microbe 2015
Coleman BI, Skillman KM, Jiang RHY, Childs LM, Altenhofen LM, et al
A
Plasmodium falciparum histone deacetylase regulates antigenic variation and
gametocyte conversion.
Cell Host Microbe 2014; Aug 13
Jiang RHY, de Bruijn I, Haas BJ, et al.
Distinctive expansion of potential virulence genes in the genome
of the oomycete fish pathogen Saprolegnia parasitica.
PLoS Genetics 2013 Jun;9(6)
in vitro human liver engineering and genomics
Roth A*, Maher SP*, Conway A ...Lanar DE, Nosten F, Davidson S, Jiang RHY, Kyle DE, Adams JH.
A comprehensive model for assessment of liver stage therapies targeting Plasmodium vivax and Plasmodium falciparum
Nature Communications 2018 PMID: 29743474
Roth A, Adapa SR, Zhang M, Liao X, Saxena V, Goffe R, Li S, Ubalee R, Saggu
GS, Pala ZR, Garg S, Davidson S, Jiang RHY^, Adams JH^.
Unraveling the Plasmodium
vivax sporozoite transcriptional journey from mosquito vector to human host.
Scientific
Report 2018 PMID:30111801
Maher SP, Conway AJ, Roth A, Adapa SR, Cualing P, ....Saadi WM, Adams JH, Jiang RHY, Kyle DE
An adaptable soft-mold embossing process for fabricating optically-accessible, microfeature-based culture systems and application toward liver stage antimalarial compound testing.
Lab-on-a-Chip 2020 PMID: 32055808